Mouse Monoclonal Antibody to GAPDH
Catalog No.：FA00028
【Specification】 Aliases : G3PD; GAPD; MGC88685;Glyceraldehyde-3-phosphate dehydrogenase Entrez GeneID : 2597 Swissprot : P04406 clone : 1A10 WB Predicted band size : 37kDa Host/Isotype : Mouse IgG1 Antibody Type: Loading Control Antibody Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. Species Reactivity: Human,Mouse,Rat,Rabbit,Monkey,Pig Immunogen : Purified recombinant fragment of human GAPDH expressed in E.Coli. Formulation: Purified antibody in PBS with 0.05% sodium azide.
【Description】 Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is well known as one of thekey enzymes involved in glycolysis. It catalyzes an important energy-yielding step incarbohydrate metabolism, the reversible oxidative phosphorylation ofglyceraldehyde-3-phosphate in the presence of inorganic phosphate andnicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer ofidentical chains. Besides its functioning as a glycolytic enzyme in cytoplasm, recentevidence suggest that mammalian GAPDH is also involved in a great number ofintracellular proceses such as membrane fusion, microtubule bundling,phosphotransferase activity, nuclear RNA export, DNA replication, and DNA repair.During the last decade a lot of findings appeared concerning the role of GAPDH indifferent pathologies including prostate cancer progression, programmed neuronalcell death, age- related neuronal diseases, such as Alzheimer. 【
Application】 WB 1/5000- 1/20000 IF 1/200 - 1/1000 IHC 1/200 - 1/1000 FCM 1/200 - 1/400 ELISA 1/10000已验证抗体 * 退换有保障 For Research Only-Time Saved, Science Advanced 苏州丰度蛋白生物技术有限公司（www.fedprotein.com) Western blot analysis using GAPDH mouse mAb against Hela (1), A549 (2), A431 (3), MCF-7 (4), K562 (5), Jurkat (6), HL60 (7), SKN-SH (8) and SKBR-3 (9) cell lysate. Confocal Immunofluorescence analysis of methanol-fixed HepG2 (left) and Hela (right) cells using GAPDH mouse mAb (green), showing cytoplasmic localization. Blue: DRAQ5 fluorescent DNA dye. Immunofluorescence analysis of NIH3T3 cells using GAPDH mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Flow cytometric analysis of NIH3T3 cells using GAPDH mouse mAb (green) and negative control (red). Flow cytometric analysis of COS7 cells using GAPDH mouse mAb (green) and negative control (red). Immunohistochemical analysis of paraffin- embedded human breast carcinoma (left) and kidney carcinoma (right), showing cytoplasmic localization using GAPDH mouse mAb with DAB staining. Immunohistochemical analysis of paraffin- embedded Mouse colon(A) Mouse pancreas(B) using GAPDH mouse mAb with DAB staining. Immunohistochemical analysis of paraffin- embedded Rat stomach using GAPDH mouse mAb with DAB staining. Immunohistochemical analysis of paraffin- embedded Rabbit pancreas(A) Rabbit prostate(B) using GAPDH mouse
