Mouse Monoclonal Antibody to HH3
Catalog No.:FA00943 【Specification】
Aliases :
HIST2H3A;H3/n; H3/o
Entrez GeneID :
333932
Swissprot:
Q71DI3
clone :
6D3B9
WB Predicted band size: 15.4kDa
Host/Isotype:
Mouse IgG1
Antibody Type:
Primary antibody
Storage:
Store at 4°C short term. Aliquot and store at -20°C long term. Avoidfreeze/thaw cycles.
Species Reactivity:
Human,Mouse,Rat
Immunogen :
Synthesized peptide fragment of human HH3 (AA: 121-136)expressed in E. Coli.
Formulation:
【Description】
Histones are basic nuclear proteins that are responsible for the nucleosomestructure of the chromosomal fiber in eukaryotes. This structure consists ofapproximately 146 bp of DNA wrapped around a nucleosome, an octamer composedof pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatinfiber is further compacted through the interaction of a linker histone, H1, with theDNA between the nucleosomes to form higher order chromatin structures. Thisgene is intronless and encodes a member of the histone H3 family. Transcripts fromthis gene lack polyA tails; instead, they contain a palindromic termination element.This gene is found in a histone cluster on chromosome 1. This gene is one of fourhistone genes in the cluster that are duplicated; this record represents thecentromeric copy.
【Application】
WB
1/500 - 1/2000
FCM
1/200 - 1/400
ELISA
1/ 10000
For Research Only-Time Saved, Science Advanced苏州丰度蛋白生物技术有限公司(www.fedprotein.com)
Black line: Control Antigen (100 ng); Purple
line: Antigen(10ng); Blue line: Antigen (50
ng); Red line: Antigen (100 ng)
Western blot analysis using HH3 mouse
mAb against K562 (1),
C6(2),HEK293(3),PC-12(4) and NIH/3T3(5)
cell lysate.
Flow cytometric analysis of NIH/3T3 cells
using HH3 mouse mAb (green) and
negative control (red).