Aliases :

LIM domain-binding protein 2, LDB-2, Carboxyl-terminal LIM domain-binding protein 1, CLIM-1, LIM domain-binding factor CLIM1, LDB2, CLIM1

Entrez GeneID :

9079

Swissprot :

O43679

WB Predicted band size :

42.8 kDa

Host/Isotype :

Rabbit IgG

Antibody Type:

Primary antibody

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Species Reactivity:

Human

Immunogen:

This LDB2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 314-342 amino acids from the C-terminal region of human LDB2.

Formulation:

Purified antibody in PBS with 0.05% sodium azide.

Description:

Genes encoding LIM domain-binding factors were initially isolated in a screen for proteins that physically interact with the LIM domains of nuclear proteins (summarized by Semina et al., 1998 [PubMed 9799849]). These proteins, such as the one encoded by the LDB2 gene, are capable of binding to a variety of transcription factors and are likely to function at enhancers to bring together diverse transcription factors and form higher order activation complexes or to block formation of such complexes (Jurata and Gill, 1997 [PubMed 9315627]). The family of genes encoding LIM domain-binding factors includes 2 members isolated from the mouse, Clim1 (Bach et al., 1997 [PubMed 9192866]) and Clim2/Lbd1/Nli (Agulnick et al., 1996 [PubMed 8918878]; Jurata et al., 1996 [PubMed 8876198]; Bach et al., 1997 [PubMed 9192866]) and their homologs cloned from the frog, chicken, and fly. The fact that LIM domain-binding factors are likely to be involved in the coordination of the transcriptional activity of many diverse factors might implicate them in human phenotypes characterized by multiple affected sites.

Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane 1-2: Hela and Human fetal brain tissue , Primary antibody: P04823(DTX1 Antibody) at dilution 1/250, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 90 seconds

The image is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using P04823(DTX1 Antibody) at dilution 1/30. (Original magnification: ×200)

The image is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using P04823(DTX1 Antibody) at dilution 1/30. (Original magnification: ×200)