Mouse Monoclonal Antibody to Cytokeratin 8
Catalog No.：FA00201
【Specification】
Aliases: CK8;CYK8;K2C8;KRT8
Entrez GeneID: 3856
Swissprot: P05787
clone : 8A5D12
WB Predicted band size: 54kDa
Host/Isotype: Mouse IgG1
Antibody Type: Primary antibody
Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. Species Reactivity: Human,Mouse
Immunogen: Purified recombinant fragment of human Cytokeratin (aa391-483) expressed in E. Coli. Formulation: Ascitic fluid containing 0.03% sodium azide.
【Description】 Cytokeratin 8, also known as CK8, KRT8, K8. Entrez Protein NP_002264. It is a member of the type II keratin family.Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells.It typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis.
【Application】 WB 1/500 - 1/2000 IHC 1/200 - 1/1000 IF 1/200 - 1/1000 ELISA 1/10000已验证抗体 * 退换有保障 For Research Only-Time Saved, Science Advanced 苏州丰度蛋白生物技术有限公司（www.fedprotein.com) Western blot analysis using CK8 mouse mAb against A549 (1), Hela (2), MCF-7 (3), A431 (4) and HepG2 (5) cell lysate. Confocal Immunofluorescence analysis of methanol-fixed ECA109 cells (left) and HepG2 cells (right) using CK8 mouse mAb (green), showing cytoplasmic localization. Blue: DRAQ5 fluorescent DNA dye. Immunohistochemical analysis of paraffin- embedded human breast carcinoma (A), lung cancer (B) and ovarian cancer tissue (C), showing membrane and cytoplasmic localization with DAB staining using CK8 mouse mAb. Immunohistochemical analysis of paraffin-embedded human Kidney tissues using CK8 mouse mAb
