Mouse Monoclonal Antibody to HSPA5
Catalog No.：FA00345
【Specification】 Aliases: BIP; MIF2; GRP78; FLJ26106; HSPA5
Entrez GeneID : 3309 Swissprot: P11021 clone : 4E3 WB Predicted band size: 78kDa Host/Isotype: Mouse IgG1 Antibody Type: Primary antibody Storage: Store at 4°C short term. Aliquot and store at -20°C long term.Avoidfreeze/thaw cycles. Species Reactivity: Human,Mouse,Rat,Monkey,Rabbit Immunogen : Purified recombinant fragment of human HSPA5 expressed in E.Coli. Formulation: Purified antibody in PBS with 0.05% sodium azide.
【Description】 When Chinese hamster K12 cells are starved of glucose, the synthesis of severalproteins, called glucoseregulated proteins (GRPs), is markedly increased.Hendershot et al. (1994) (PubMed 8020977) pointed out that one of these, GRP78(HSPA5), also referred to as 'immunoglobulin heavy chain-binding protein' (BiP), isa member of the heat-shock protein-70 (HSP70) family and is involved in the foldingand assembly of proteins in the endoplasmic reticulum (ER). Because so many ERproteins interact transiently with GRP78, it may play a key role in monitoringprotein transport through the cell.Probably plays a role in facilitating the assemblyof multimeric protein complexes inside the ER.The HSP70 proteins are ubiquitousmolecular chaparones that are found in all organisms and tissue types. Like othermembers of the HSP70 family, BiP is a peptide-binding ATPase that is able todifferentiate native proteins from unfolded polypeptides. BiP does not bind to fullyfolded and assembled proteins, except in the presence of other co-chaparones. BiPis involved in a number of key mechanisms and pathways including polypeptidetranslocation across the endoplasmic reticulum, folding, assembly, transport ofsecreted or membrane proteins, and the regulation of calcium homeostasis.Although BiP is relatively abundant, marked increases in BiP occur where there isan accumulation of unfolded polypeptides. For this reason, BiP has been identifiedas a marker for various disease states that are associated with secretory andtransmembrane protein misfolding.已验证抗体 * 退换有保障 For Research Only-Time Saved, Science Advanced 苏州丰度蛋白生物技术有限公司（www.fedprotein.com)
【Application】 WB 1/500 - 1/2000 IHC 1/100 - 1/500 IF 1/50 - 1/500 FCM 1/200 - 1/400 ELISA 1/10000 Western blot analysis using HSPA5 mouse mAb against NIH/3T3 (1), Hela (2) and Jurkat (3) cell lysate. Western blot analysis using HSPA5 mouse mAb against Mouse brain(1)PC- 12(2)F9(3)COS-7(4) cell lysate. Immunofluorescence analysis of COS7 cells using HSPA5 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Flow cytometric analysis of COS7 cells using HSPA5 mouse mAb (green) and negative control (red). Flow cytometric analysis of Hela cells using HSPA5 mouse mAb (green) and negative control (red). Flow cytometric analysis of NIH/3T3 cells using HSPA5 mouse mAb (green) and negative control (red).已验证抗体 * 退换有保障 For Research Only-Time Saved, Science Advanced 苏州丰度蛋白生物技术有限公司（www.fedprotein.com) Flow cytometric analysis of PC-12 cells using HSPA5 mouse mAb (green) and negative control (red). Immunohistochemical analysis of paraffin- embedded human Thyroid tissues using HSPA5 mouse mAb Immunohistochemical analysis of paraffin- embedded Mouse cerebellum using HSPA5 mouse mAb with DAB staining. Immunohistochemical analysis of paraffin- embedded Rat brain using HSPA5 mouse mAb with DAB staining. Immunohistochemical analysis of paraffin- embedded Rat kidney using HSPA5 mouse mAb with DAB staining. Immunohistochemical analysis of paraffin- embedded Rabbit kidney using HSPA5 mouse mAb with DAB staining. Immunohistochemical analysis of paraffin- embedded Rabbit spinal cord using HSPA5 mouse mAb with DAB staining.
