Mouse Monoclonal Antibody to CTNNB1
Catalog No.：FA02043 【Specification】
Aliases : CTNNB; MRD19; armadillo;Beta-catenin
Entrez GeneID :
1499
Swissprot :
P35222
clone :
5B6E9
WB Predicted band size :
85.5kDa
Host/Isotype :
Mouse IgG1
Antibody Type:
Primary antibody
Storage:
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity:
Human, Mouse, Rat, Monkey
Immunogen :
Purified recombinant fragment of human CTNNB1 (AA: 632-781)expressed in E. Coli.
Formulation:
【Description】
The protein encoded by this gene is part of a complex of proteins that constituteadherens junctions (AJs). AJs are necessary for the creation and maintenance ofepithelial cell layers by regulating cell growth and adhesion between cells. Theencoded protein also anchors the actin cytoskeleton and may be responsible fortransmitting the contact inhibition signal that causes cells to stop dividing once theepithelial sheet is complete. Finally, this protein binds to the product of the APCgene, which is mutated in adenomatous polyposis of the colon. Mutations in thisgene are a cause of colorectal cancer (CRC), pilomatrixoma (PTR), medulloblastoma(MDB), and ovarian cancer. Alternative splicing results in multiple transcriptvariants.
【Application】
WB
1/500 - 1/2000
IHC
1/200-1/ 1000
FCM
1/200-1/400
ELISA
1/ 10000
For Research Only-Time Saved, Science Advanced苏州丰度蛋白生物技术有限公司（www.fedprotein.com)
Black line: Control Antigen (100 ng);Purple line:
Antigen (10ng); Blue line: Antigen (50 ng); Red
line: Antigen (100 ng)
Western blot analysis using CTNNB1 mouse mAb against Hela (1), HepG2 (2),NIH3T3
(3),MCF-7 (4), C6 (5),COS-7 (6),K562
(7),Jurkat (8), A549 (9),SH-SY5Y (10),BEL- 7402 (11), HEK293 (12), and HEK293-6e (13)
cell lysate.
Flow cytometric analysis of HepG2 cells using
CTNNB1 mouse mAb (green) and negative
control (red).
Flow cytometric analysis of A549 cells using
CTNNB1 mouse mAb (green) and negative
control (red).
Immunohistochemical analysis of paraffin-
embedded human cervical cancer tissues using
CTNNB1 mouse mAb with DAB staining.
Immunohistochemical analysis of paraffin-
embedded human rectal cancer tissues using
CTNNB1 mouse mAb with DAB staining.
